This new research out of China, led by scientists from Procter & Gamble Company and the Single-Cell Center, Qingdao Institute of BioEnergy and Bioprocess Technology of the Chinese Academy of Sciences, was published in the journal mBio on Mar. 9. The researchers studied 40 participants whose oral hygiene routine was interrupted over the course of a month. They found that in between 24 to 72 hours of abandoning their normal routine, there was a steep decrease in the amount of “good oral bacteria” (rothia species) along with the anti-inflammatory chemical betaine. And for more oral hygiene advice, check out This Is What Happens to Your Body When You Don’t Floss Your Teeth. As the good bacteria decreased, there was simultaneously a sharp rise in the amount of “bad bacteria”—specifically multiple salivary cytokines, which are the proteins and other molecules produced by immune system cells and connected with inflammation in the body. The researchers also found significant quantities of the kind of bacteria usually found in patients with peridontitis (the gum infection that can ultimately lead to the loss of teeth), even before any symptoms of this condition were present. And for another tip on keeping your teeth healthy, check out How Often You Should Really Change Your Toothbrush, Dentists Say. In addition to these bacterial changes, after just one day of not brushing your teeth, actual physical damage starts to occur, too. “Plaque functional profiles already resemble that of the severe gingivitis stage within 24 [hours],” the study authors write. Shafer’s Textbook of Oral Pathology warns that built up plaque starts to decalcify the lower layer of dentin, the protective material that sits below the surface enamel. Once this is penetrated, damage can start to occur to the teeth, which can’t be reversed by resuming regular brushing. Unlike enamel, dentin also has nerves running through it, meaning any damage to it is more likely to result in pain. And for more health news delivered right to your inbox, sign up for our daily newsletter.ae0fcc31ae342fd3a1346ebb1f342fcb After the initial sharp change in the bacterial makeup of study participants’ mouths, researchers noticed more damage occurring. “We also found a sudden ‘aging’ of the bacteria in the mouth,” the study’s senior author XU Jian, director of Single-Cell Center, said in a statement. “Their oral microbiome had aged the equivalent of about a year in less than a month.” Previous studies have shown that a person’s oral microbiology can be given an “age” as the amount of healthy rothia typically declines naturally with the passing of time. A separate 2019 study in the European Journal of Preventive Cardiology also found a possible link between poor oral hygiene and heart disease. So the message is clear—even if you’re not leaving the house or seeing many people lately, brushing your teeth is something you shouldn’t be cutting corners on, even for 24 hours. Of course, you know twice a day is key, but Here’s What Happens When You Only Brush Your Teeth Once a Day.